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Recognition of amine nucleophiles by AdmF. FT mass spectrum (10+ charge state) of the AdmF reaction products arising from the isopeptide formation between the acyl chain of butyryl-S-AdmA and aminoacyl-S-AdmI. AdmI was loaded with the amine donors (S)-β-Phe, (R)-β-Phe, d-Phe, l-Phe, and (S)-β-Ala (top to bottom). All reactions were quenched after 30 min of incubation at 30 °C.
