Figure 6. Effects of CGRP on spinal ERK1/2 and Akt phosphorylation.
In A-D, S1 spinal cord was transversely sectioned at a thickness of 250 μm and incubated with CGRP (250 nM) for a designated time period indicated in A and C. Results showed that exogenous CGRP was able to increase Akt activation (D) but not ERK1/2 activation (B) in the spinal cord slices at 20 min of incubation (n=3 independent experiments).