Figure 8.—

Amun overexpression downregulates Achaete levels in a cell-autonomous manner. (A and B) Nota from 9-hr APF pupae (27°) stained with anti-Achaete antibody (green). (A, A′, and A″) A sr-Gal/UAS-myr-mRFP notum. RFP (red) reflects expression of mRFP within the sr domain. Achaete-positive cells (arrows) mark the microchaeta proneural groups during this developmental stage. (B, B′, and B″) A sr-Gal4/UAS-Amun∷RFP notum shows a severe reduction or absence of Achaete expression (arrowheads) within the sr expression domain where Amun∷RFP is overexpressed. (C, C′, and C″) A 6- to 7-hr APF notum stained with anti-Achaete antibody (green in C and C″). Amun∷RFP is overexpressed randomly throughout the notal disc in Act5C-Gal4 clones (red). Achaete protein levels are downregulated in cells in which Amun∷RFP is overexpressed (arrowheads). Achaete-positive cells can be found directly adjacent to Amun∷RFP-positive cells (arrows) in clones in all regions of the notum.