Figure 4.—

The Pan2 mRNA deadenylase is dispensable for septin organization. (A) Upper panel: Cells were treated with or without 0.2 m HU for 16 hr and processed for microscopy as in Figure 2A. Lower panel: Immunofluorescence with the anti-Cdc11 antibody in pan2Δ cultures, with or without treatment with 0.2 m HU for 16 hr. (B) Double pan2Δ ccr4-1 mutants were either from log phase cultures (−HU) or after treatment with 0.2 m HU for 16 hr. Cells were processed for microscopy and immunofluorescence as in Figures 2 and 3. The arrows indicate the big elongated cells that did not stain for septins, as well as cells displaying bud chains and ectopic septin rings.