Figure 4.—

Effects of mutant forms of lacI–HP1 on reporter gene expression. Heat-shock-induced expression of hsp26-tag and hsp70-white in the presence or absence of LacI–HP1 or GFP–LacI (negative control). (A) Graphical representation of results from three independent Northerns for hsp70 inserted at three different cytological locations: 4D5 (solid bars), 79D1 (dark shaded bars), and 87C1 (light shaded bars). Percentage of relative expression was determined by setting the expression value for the reporter gene without tethered HP1 at 100% (-, open bar). Error bars represent standard error of the mean. (B) Graphical representation of results obtained from three independent Northerns for hsp26. Designations and calculations were the same as described for B.