Figure 6.—

Loss of dSETDB1 affects silencing by tethered HP1. (A) Chromosomes were fixed, squashed, and stained with antibodies to LacI and dSETDB1. (B) Graphical representation of results obtained from four independent RT–PCR experiments for hsp26 upon tethering of HP1 in the presence or absence of dSETDB1 (wt and mutant, respectively). Expression from the transgene with no tether (−) or with GFP–LacI serve as controls. Error bars represent standard error of the mean. (C) Same as in B except primers for the hsp70 reporter were used for RT–PCR analysis.