Fig. 2.

Dot/Icm dependent translocation of LegK1 into U937 cells. Differentiated U937 cells were infected with wild-type L. pneumophila (Lp02) or the dotA mutant (Lp03) strain harboring TEM1-LegAS4, TEM1-LegK1, or TEM1-GST expression plasmids at a MOI of 10. Two hours after infection, cells were loaded with CCF2/AM dye and translocation was determined by a comparison of cleaved to uncleaved CCF2/AM that gives blue and green fluorescence, respectively. The fluorescence images shown are representatives of three separate experiments.