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. 2009 Aug 10;106(33):13915–13920. doi: 10.1073/pnas.0905780106

Fig. 3.

Fig. 3.

PS58-induced silencing is rapid, persistent, and occurs with high efficacy. (A) DM500 myotubes were mock-treated or treated with PS58. RNA was isolated 2–48 h after start of transfection. Northern blot analysis indicated that expanded hDMPK was degraded within 2 hours. A representative blot is shown. The means of 3 experiments are summarized in the Bottom panel (P < 0.05). (B and C) DM500 myoblasts and myotubes in different stages of myogenesis were treated with a single 200-nM PS58 dose or mock-treated and then cultured for up to 10 days. Northern blot analysis demonstrated that hDMPK RNA remained undetectable for up to 10 days. (D) Concentration-response curve in DM500 myotubes. Data points are the mean of at least 3 measurements.