Fig. 5.

Immunoprecipitation of actively translated Prm1 mRNA from Rpl22^ha-expressing homozygous mouse testis. (A) qRT-PCR Taqman assay using a probe specific for Prm1 mRNA comparing input (total RNA) and HA-immunoprecipitated (polysome-associated RNA) from P25, P28, and P32 Rpl22^ha-expressing homozygous mouse testis homogenates. qRT-PCR analysis of beta-actin (Actb) transcripts was performed as a control. Values are the mean ± SEM of three independent experiments. (B) Table showing the percentage of polysome-associated transcripts compared to total transcripts (% Translated) of Prm1 and Actb at each time point. (C) Coomassie Brilliant Blue staining showing basic proteins isolated from nuclear pellets of P25, P28, and P32 Rpl22^ha-expressing homozygous mouse testis. Samples were resolved on 15% acrylamide acid-urea gels. (D) Northern blot analysis revealing the predominance of partially deadenylated Prm1 transcripts in the polysome-associated samples.