Fig. 4.

Induction of a Mac-1 activation-specific neoepitope involves CD14. BMDM from C57BL/6 or CD14^−/− mice were exposed to wt, ΔrmlD or ΔbclA spores and then stained with the anti-Mac-1 mAb (clone CBRM1/5) directed to an activation epitope or an isotype-matched mAb. Samples were then washed and analyzed by flow cytometry. Tinted histograms correspond to cells stained with irrelevant mAb and blank histograms correspond to cells stained with specific mAb. Each of such histograms in this figure and in Fig. 5 and Fig. 5C have been scaled to 100% maximum in Flowjo.