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. 2009 Aug 4;106(33):13968–13973. doi: 10.1073/pnas.0906367106

Fig. 1.

Fig. 1.

H. polygyrus-induced Th2 cell differentiation, effector function and memory responses are not altered in TSLPR−/− mice. (A) mRNA expression of IL-4 as determined by quantitative real-time RT-PCR using purified CD4+ T cells from the draining MLN of naïve or H. polygyrus infected TSLPR−/− (black bars, n = 4–6) or C57BL/6 (open bars, n = 4–6) mice. Cells were harvested 11 days after primary (1°) or secondary (2°) helminth infection. The graph represents the combined data of two independent experiments. (B) The percentage of total white blood cells that are basophils in 1° (circles) or 2° (squares) H. polygyrus-infected TSLPR−/− (closed symbols, n = 6) or C57BL/6 (open symbols, n = 5) mice. (C and D) Worm (C) and granuloma counts (D) in the intestine of TSLPR−/− (black symbols, n = 5 to 6) or C57BL/6 (open symbols, n = 5) mice, 11 days after primary (1°, circles) or secondary (2°, squares) infection with H. polygyrus. Horizontal bars represent the mean value. ELISA determination of total IgE (E), total IgG1 (F), HES-specific IgG1 (G), and HES-specific IgG2a (H) levels in the serum of TSLPR−/− (closed symbols, n = 6) or C57BL/6 (open symbols, n = 5) mice subjected to 1° (circles) or 2° (squares) H. polygyrus infection. B.D., below detection limit. No impact of H. polygyrus infection on total IgG2a levels could be noted. Data represent means ± SD of one experiment and are representative of three independent experiments.