Fig. 4.

Development of CD4⁺CD25⁻LAG3⁺ T cells. (A) Flow cytometry of LAG-3 and CD25 expression in the thymus and spleen of OT-II TCR transgenic mice with or without the RIP-mOVA transgene. Upper and Lower plots are gated on CD4⁺Vβ5.1/5.2⁺ and CD4⁺CD25⁻Vβ5.1/5.2⁺ T cells, respectively. Representative FACS dot plots from three independent experiments are shown. (B) Flow cytometry of LAG-3 and CD45RB expression in the spleen and Peyer's patch (PP) of specific-pathogen-free (SPF) (Left) and germfree (GF) (Right) mice gated on CD4⁺CD25⁻ T cells. Representative FACS dot plots from at least three independent experiments are shown.