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. 2009 Jul 30;106(33):13998–14003. doi: 10.1073/pnas.0810087106

Fig. 1.

Fig. 1.

Pancreatic islet and peripheral tissue inflammation in the type 2 diabetic GK rat. (A) Total RNA was extracted from 2-month-old male Wistar and GK freshly isolated rat islets, and real-time PCR was performed for the indicated genes and normalized to a housekeeping gene (18S). Shown in parentheses are the fold increases in GK islets vs. Wistar controls. Casp-1, caspase-1. (B) Wistar and GK rat islets were isolated and cultured for 48 h at 20 islets/dish, and conditioned media were assayed for the indicated cytokine/chemokine. Data were normalized for total islet protein. Data represent 3 different (A) and 4 different (B) islet isolations with islets pooled from 2–3 animals each time and experiments performed in triplicate. (C) Real-time PCR was performed on cDNA from liver, adipose, and muscle tissue from 2-month-old male Wistar and GK rats. Data are representative of 4 animals per strain and are shown as fold increases vs. Wistar controls. *, P < 0.05 as determined by Student's t test.