Fig. 2.

IL-1Ra inhibits GK islet cytokine and chemokine release in vitro. Isolated Wistar and GK islets were plated at 20 islets/well and treated in vitro without (−) or with 1000 ng/mL IL-1Ra (+) for 48 h. Thereafter, conditioned media were removed and assayed for (A) IL-6, (B) chemokine KC, (C) MCP-1, and (D) MIP-1α, and data were normalized for total islet protein. Data are presented relative to GK untreated. Data represent 3 different islet isolations with islets pooled from 2–3 animals each time and experiments performed in quadruplicate. *, P < 0.05 as determined by ANOVA with Newman–Keuls posthoc analysis.