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. 2009 Jul 30;106(33):13998–14003. doi: 10.1073/pnas.0810087106

Fig. 4.

Fig. 4.

IL-1Ra treatment reduces tissue inflammation in the GK rat. (A) Pancreatic islets were isolated from GK rats following treatment with IL-1Ra by twice daily s.c. injections [GK saline, n = 6; GK IL-1Ra (50 mg/kg/injection), n = 5]. Total RNA was extracted from isolated islets and real-time PCR was performed for the indicated genes, normalized to 18S, and expressed relative to GK saline controls. (B and C) Immunohistochemistry was performed for CD68, MHC class II, CD53, and granulocytes associated with islets and quantified (n = 3 for both treatment groups). (D) β cell area/total pancreatic area was quantified by immunohistochemistry (n = 3 for both treatment groups). (E) Real-time PCR was performed on cDNA samples from liver, adipose, and muscle tissue from GK saline- (n = 4) and IL-1Ra-treated animals (n = 5). n represents the number of animals analyzed. *, P < 0.05 as determined by Student's t test.