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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: In Vitro Cell Dev Biol Anim. 2008 Dec 31;45(3-4):163–174. doi: 10.1007/s11626-008-9159-0

Table 1. Percentage change in ZSSJ and RTS34st cell numbers 2 weeks after different doses of gamma-irradiation.

Irradiation ZSSJ at 26 °C RTS34st at rt RTS34st at 26 °C
none + 1083.81 ± 69.78 + 1377.69 ± 43.88 - 30.44 ± 8.21
0.5 Gy + 1126.38 ± 131.46 + 1392.79 ± 28.70 - 31.18 ± 5.80
10 Gy + 675.34 ± 19.91 + 565.87 ± 22.12 - 63.03 ± 2.42
20 Gy + 40.72 ± 4.40 - 21.28 ± 5.59 -
30 Gy - 48.07 ± 6.76 - 54.71 ± 2.59 - 72.99 ± 2.83
40 Gy - 66.54 ± 1.74 - 59.58 ± 4.80 -
50 Gy - 71.11 ± 5.31 - 70.89 ± 1.77 - 71.29 ± 1.26

RTS34st and ZSSJ cells were irradiated in 25 cm2 tissue culture flasks and reseeded into 12-well tissue culture plates at 5.0 × 104 cells per well and incubated at either room temperature (rt) or 26 °C. At 24 h (day 0) and 2 weeks after plating, a Coulter particle counter was used to determine cell number in triplicate wells for each treatment. The counts at 2 weeks were expressed as a percentage of the day 0 count.