Table 1. Percentage change in ZSSJ and RTS34st cell numbers 2 weeks after different doses of gamma-irradiation.
Irradiation | ZSSJ at 26 °C | RTS34st at rt | RTS34st at 26 °C |
---|---|---|---|
none | + 1083.81 ± 69.78 | + 1377.69 ± 43.88 | - 30.44 ± 8.21 |
0.5 Gy | + 1126.38 ± 131.46 | + 1392.79 ± 28.70 | - 31.18 ± 5.80 |
10 Gy | + 675.34 ± 19.91 | + 565.87 ± 22.12 | - 63.03 ± 2.42 |
20 Gy | + 40.72 ± 4.40 | - 21.28 ± 5.59 | - |
30 Gy | - 48.07 ± 6.76 | - 54.71 ± 2.59 | - 72.99 ± 2.83 |
40 Gy | - 66.54 ± 1.74 | - 59.58 ± 4.80 | - |
50 Gy | - 71.11 ± 5.31 | - 70.89 ± 1.77 | - 71.29 ± 1.26 |
RTS34st and ZSSJ cells were irradiated in 25 cm2 tissue culture flasks and reseeded into 12-well tissue culture plates at 5.0 × 104 cells per well and incubated at either room temperature (rt) or 26 °C. At 24 h (day 0) and 2 weeks after plating, a Coulter particle counter was used to determine cell number in triplicate wells for each treatment. The counts at 2 weeks were expressed as a percentage of the day 0 count.