Table 3.

Characteristics of gene-specific qPCR assays

Gene name (synonym)	Genebank Access n° Gene ID	Gene location	Primer sequence 5'-3'	Amplicon size	PCR efficiency
ACTB actin, beta	NM_001101 60	7p15-p12	f: tggctggggtgttgaaggtct r: agcacggcatcgtcaccaact	238	1.98
ALAS1 aminolevulinate delta synthase 1, transcript variant 1	NM_000688 211	3p21.1	f: aacttgccaaaatctgtttc r: ggtgatgagggagtctgaat	159	1.99
B2M beta-2-microglobulin	NM_004048 567	15q21-q22.2	f: cagcgtactccaaagattca r: gaatgctccactttttcaat	240	1.95
GAPDH glyceraldehyde-3-phosphate dehydrogenase	NM_002046 2597	12p13	f: tgaacgggaagctcactgg r: tccaccaccctgttgctgta	307	1.90
HMBS hydroxymethylbilane synthase, transcript variant 1	NM_000190 3145	11q23.3	f: gaaagacaacagcatcatgag r: accaaggagcttgaacatgc	145	1.98
HPRT1 hypoxanthine phosphoribosyltransferase 1	NM_000194 3215	Xq26.1	f: ctgacctgctggattaca r: gcgaccttgaccatcttt	256	1.90
K-ALPHA tuba1a, tubulin	NM_006082 10376	12q13.12	f: cagatgccaagtgacaagac r: tccaacacaaggtcaatgat	257	1.94
RPL27 ribosomal protein L27	NM_000988 6155	17q21.1-q21.2	f: tcgccaagagatcaaagataa r: ctgaagacatccttattgacg	121	1.94
RPS18 ribosomal protein S18	NM_022551 6222	6p21.3	f: agcttgttgtccagaccatt r: tgaggaaagcagacattgac	187	1.84
RPS29 ribosomal protein S29 transcript variant 2	NM_001030001 6235	14q	f: gcactgctgagagcaagatg r: ataggcagtgccaaggaaga	213	1.95
SDHA succinate dehydrogenase complex, subunit A, flavoprotein	NM_004168 6389	5p15	f: agcaagctctatggagacct r: taatcgtactcatcaatccg	200	1.80
TBP TATA box binding protein	NM_003194 6908	6q27	f: cacgaaccacggcactgatt r: ttttcttgctgccagtctggac	89	2.01