TABLE VI.

ADENYLATE CYCLASE, NANOMOLES PER MILLIGRAM PROTEIN HOMOGENATE PER 15 MINUTES

			Glucagon stimulated					NaF stimulated, 10 mM.
Group	No.	Basal, H2O	10−9M	10−8M	10−7M	10−6M	10−5M
Normal	18	18.6±4.8	40.3±12.6	59.2±13.2	77.2±17.2	93±21.8	97±24.2	126±39.3
4	3	33.3±14.9	75.9±18.1	105±45.6	179.4±96.3	287±128	238.6±104.5	352±128.6
pValue		NS	NS	NS	NS	NS	<0.05	<0.05
5	5	8.8±4.6	14.1±6.9	24.9±16.7	35.3±19.9	53.2±36.3	57.2±48.9	81.6±54.7
p Value		<0.01	<0.001	<0.001	<0.001	<0.01	<0.02	NS
6	5	24±6.6	37.5±8.7	54.4±12.5	76.8±24	111.4±25.5	127.9±31.2	222±71.5
p Versus normal		NS	NS	NS	NS	NS	<0.05	<0.05
p Versus group 5		<0.01	<0.01	<0.02	<0.02	<0.02	<0.05	<0.01
7	2	15.5±10.6	16.8±10.8	31.4±29.1	40.6±37.4	61±48	66.6±49	120.2±113
p Versus normal		NS	<0.05	<0.02	<0.02	NS	NS	NS
p Versus Group 5		NS	NS	NS	NS	NS	NS	NS
8	2	11.5±6.3	13.5±9.1	20.3±4.3	24.6±7.4	44.4±17.5	42.5±14.5	82.8±32.2
p Versus normal		NS	<0.01	<0.001	<0.001	<0.01	<0.01	NS
p Versus group 5		NS	NS	NS	NS	NS	NS	NS

In each group, the test group is compared by the Students t test with the normal dogs. In groups 6, 7 and 8, a second companion, lower p value, is made with the eviscerated dogs of group 5 which did not receive hormones.

NaF, Sodium fluoride; NS, not significant.