Figure 3.

ER-α/-β protein expression in immortalized bone marrow stromal cells. Nuclear extracts were isolated from immortalized stromal cells from the propositus (P) (lanes 2 and 4) and an age-matched 28-yr-old female donor (C) (lanes 1 and 3) (14). Nuclear extract protein preparations, equalized for protein content, were analyzed by SDS-PAGE and Western blot. The samples were probed with antisera against amino terminal sequence of ER-α using ER21 (lanes 1 and 2) or to ER-β using PAI-310 (lane 3 and 4) as described in Patients and Methods.