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. Author manuscript; available in PMC: 2009 Aug 19.
Published in final edited form as: Fungal Genet Biol. 2008 Jan 26;45(6):861–877. doi: 10.1016/j.fgb.2008.01.001

Fig. 2. Transcriptional analyses of VPS1 in the tetR-VPS1 strains.

Fig. 2

A Northern blot was probed with a 32P-labeled PCR product amplified from strain SC5314 using primers VPS1-5NB and VPS1-3NB (Table 2). There is complete repression of the VPS1 transcript in the tetR-VPS1 strains when doxycycline is present. RT-PCR on mRNA isolated from the same total RNA used in the Northern blot also showed complete repression of VPS1 transcription in the tetR-VPS1 strains in the presence of doxycycline. An ethidium bromide stained gel shows normalized loading of the RNA samples used in the Northern blot.