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. Author manuscript; available in PMC: 2009 Aug 19.
Published in final edited form as: Fungal Genet Biol. 2008 Jan 26;45(6):861–877. doi: 10.1016/j.fgb.2008.01.001

Fig. 3. Characterization of growth of the tetR-VPS1 strain.

Fig. 3

Growth of the tetR-VPS1 mutant strain was tested in the presence of sub-inhibitory concentrations of fluconazole. In complete synthetic medium supplemented with uridine at 37 °C, growth of the tetR-VPS1 strain was similar to the parental strain THE1 (A). However, growth of the tetR-VPS1 strain was inhibited in the presence of 10 μg ml-1 fluconazole (B). This sensitivity to fluconazole was increased when transcription of VPS1 was completely repressed with the addition of doxycycline. Growth curves were performed in triplicate, and corresponding error bars are indicated. The differences in growth between strain tetR-VPS1 without doxycycline and with doxycycline is statistically significant (* p < 0.0001). Similarly, the differences in growth between strain tetR-VPS1 without doxycycline and THE1-CIp10 is statistically significant (§ p < 0.0001). Statistical significance was tested by fitting growth curves to lognormal models which yielded R-squares of 99%.