Fig. 2.

NHR-25 knockdown causes male tail abnormalities. (A) Lineage diagram of the posterior seam cells V5, V6 and T, which produce the nine male-specific sensory rays. Blue circles represent cells that fuse with the hyp7 syncytium; green rectangles indicate male tail seam cells. Dotted brackets denote ray cell groups that generate individual rays 1 to 9, numbered from anterior to posterior. X represents programmed cell death. (B) Wild-type male tail. (C) Severe male abnormal (Mab) phenotype with most rays absent upon systemic nhr-25 RNAi. (D) scm::nhr-25(RNAi) males display eight major classes of Mab phenotypes. Arrows indicate defective rays or abnormal ray positions. Some individuals often displayed combinations of distinct ray phenotypes. Individual rays were distinguished on the basis of morphological attributes, such as shape, thickness, and length, and according to their position relative to the other rays.