Fig. 2.

Depletion of coronin 2A impairs cell motility, but does not affect lamellipodial dynamics. (A) MTLn3 cells were infected with lentivirus that expressed shRNAs against a non-specific sequence (shNS), coronin 2A (shCoro2A) or shCoro2A that also co-expressed human Coro2A-GFP (Rescue). Lysates were blotted for coronin 2A and for tubulin as a loading control. (B) Time-lapse microscopy of MTLn3, shNS, shCoro2A and Rescue cell lines was used to determine single-cell speed, depicted in graph. Error bars represent 95% confidence intervals. ^*P<0.0001 by Student's t-test. See supplementary material Movie 1. (C) Representative kymograph of a protruding MTLn3 cell. (D) Protrusion rate, protrusion distance and persistence were calculated from the time (y-axis) and protrusion distance (x-axis) measured from kymographs. Error bars represent 95% confidence intervals.