Skip to main content
. 2009 Aug 4;122(17):3061–3069. doi: 10.1242/jcs.051482

Fig. 4.

Fig. 4.

Depletion of coronin 2A increases P-cofilin levels, but has no effect on other focal-adhesion-turnover protein components. (A) Representative immunoblots of MTLn3, shNS, shCoro2A and Rescue cell lysates for talin, P-FAK, FAK, P-MLC, MLC, P-cofilin, cofilin, P-LIMK, LIMK and tubulin (loading control). Asterisk indicates calpain-induced talin cleavage product. (B) Quantification of P-cofilin versus total cofilin blots. Error bars represent 95% confidence intervals. (C) Representative ratiometric images of P-cofilin (P-Cof) and cofilin (Cof) for shNS and shCoro2A cells. Images were normalized to neighboring uninfected cells. Scale bars: 5 μm. (D) Quantification of ratiometric images in C. *P=0.0001 by Student's t-test. Error bars represent 95% confidence intervals.