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. 2009 Aug 4;122(17):3061–3069. doi: 10.1242/jcs.051482

Fig. 6.

Fig. 6.

Active mutants of Slingshot increase focal-adhesion disassembly and partially bypass decreases in focal-adhesion disassembly caused by depletion of coronin 2A. (A) SSH1L-GFP localizes to focal adhesions in cells expressing shNS or shCoro2A. Cells were co-stained with vinculin antibodies. Scale bars: 5 μm. (B) SSH1L-2SA-GFP localizes to focal adhesions in cells expressing shNS or shCoro2A. Cells were co-stained with vinculin antibodies. (C) Focal-adhesion disassembly rates, carried out as for Fig. 3, visualized by SSH1L-GFP or SSH1L-2SA-GFP (active) in cells expressing either shNS or shCoro2A. Error bars represent 95% confidence intervals.