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. 2009 Aug 11;122(17):3093–3103. doi: 10.1242/jcs.051813

Fig. 3.

Fig. 3.

BLM prevents RAD54-RAD51 complex formation in vitro and in vivo. (A) Purified proteins used to test the BLM(1-212)-mediated prevention of RAD51-RAD54 complex formation. (B,C) BLM prevents RAD54-RAD51 interaction. Interaction between bound GFP-RAD54 and soluble His-RAD51 was determined in the absence or presence of soluble BLM(1-212) or GST. The levels of the respective proteins after interaction were assessed by western blotting (B). Soluble BLM(109-212) was also used (C). (D) BLM prevents RAD51-RAD54 complex formation in vivo. Immunoprecipitation was carried out on the nuclear extract from BS/A-15 cells with antibodies against RAD51 or RAD54. Western blots were then performed with same antibodies. (E) BLM decreases colocalization between RAD51 and RAD54. Immunofluorescence of BS/A-15 cells with antibodies against RAD51 and RAD54. Scale bars: 5 μm.