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. 2009 Aug 11;122(17):3093–3103. doi: 10.1242/jcs.051813

Fig. 5.

Fig. 5.

BLM stimulates RAD54 function. (A) The N-terminal 1-212 amino acids of BLM enhance RAD54-mediated ATPase activity. ATPase activity was assessed with different combinations of proteins, as indicated. The concentrations of each protein are indicated in the Materials and Methods. *P<0.05 compared with the ATPase activity obtained by RAD54 alone. (B) Loss of ATPase activity in BLM(1-1417) K695A mutant. ssDNA-dependent ATPase activity was assessed using BLM(1-1417) or BLM(1-1417) K695A. (C) ATPase-dead mutant of BLM stimulates RAD54-mediated ATPase activity. BLM(1-1417) K695A was used to determine its effect on RAD54-mediated ATPase activity. (D) Schematic diagram of G5E4 array (Zhang et al., 2007). Representative REA assays with RAD54 alone (±ATP), RAD54+BLM(1-212) (+ATP) and BLM(1-212) alone (+ATP). (E) Quantification of results in D and of REA assays involving RAD54 and BLM(1-1417) K695A (+ATP).