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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Microbiol. 2009 Jul 16;73(3):466–481. doi: 10.1111/j.1365-2958.2009.06785.x

Fig. 4.

Fig. 4

The 52 kDa complex contains one ExbD and one TonB. Strains expressing chromosomally-encoded (GM1) or plasmid-encoded wild-type ExbD in the presence (KP1392/pKP660) or absence (KP1503/pKP660) of TonB were crosslinked with formaldehyde as described in Materials and Methods. Plasmids encoding ExbD also encoded wild-type ExbB and were induced with 0.0004% (w/v) L-arabinose. Samples were resolved on a 13% SDS-polyacrylamide gel and immunoblotted. A. ExbD visualized with ExbD-specific polyclonal antibodies. B. TonB visualized with TonB-specific monoclonal antibodies. Positions of molecular mass standards are indicated on the left. Identities of ExbD- or TonB-specific crosslinked complexes and monomers are indicated on the right. Light exposures for comparison of monomer levels are present at the bottom of each figure. (*) indicates an unidentified complex.