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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Mol Microbiol. 2009 Jul 16;73(3):466–481. doi: 10.1111/j.1365-2958.2009.06785.x

Fig. 6.

Fig. 6

ExbD(D25N) and TonB(H20A) fractionate identically to the wild-type forms of each protein. Strains expressing chromosomally-encoded ExbD and TonB (W3110), ExbD(D25N) (RA1021/pKP1064), and TonB(H20A) (KP1344/pKP381) were fractionated using sucrose density gradient fractionation, as described in Materials and Methods. No inducer was needed for ExbD(D25N). TonB(H20A) was induced with .00025% (w/v) arabinose. Samples were resolved on 13% SDS-polyacrylamide gels and immunoblotted. ExbD and TonB were visualized with ExbD-specific polyclonal or TonB-specific monoclonal antibodies, respectively.