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. 2009 Aug 19;106(36):15326–15331. doi: 10.1073/pnas.0903704106

Fig. 3.

Fig. 3.

Cleavage-resistant OPN mutant, OPN D135A/D157A, suppresses Hyp/RO-induced cell death. (A) Suppression of Hyp/RO-induced cell death by OPN WT and OPN DM. HeLa/pcDNA3, HeLa/OPN WT, and HeLa/OPN DM stable cells were exposed to Hyp (12 h)/RO (18 h). Cells were then analyzed by FACS and the population of SubG1 cells (line) is indicated. (B) Cleavage-defective OPN DM suppresses Hyp/RO-induced cell death. HeLa cells were transfected with pcDNA3, pOPN WT, or pOPN DM expression vector and then left untreated or exposed to Hyp (12 h)/RO for the indicated times. Cell death was determined as in Materials and Methods (Left). Values indicate mean values ± SD; (n = 3). **, P < 0.001. Expression level of plasmid-encoded OPN-HA was examined with Western blot analysis using anti-HA antibody (Right). (C) OPN DM is more tolerant to degradation than OPN during Hyp/RO. HeLa/OPN WT and HeLa/OPN DM cells were exposed to Hyp (12 h)/RO for the indicated times in the presence of 1 μM MG132 and cell extracts were analyzed with Western blotting using anti-OPN antibody.