Fig. 6.

Differential induction of OPN in tumor cells affects viability through regulation of AKT and caspase-9. (A) Different sensitivity of tumor cells to Hyp/RO-induced cell death. Cells were incubated under Hyp (12 h)/RO (18 h) and cell death was examined using EthD staining. Bars, mean values ± SD; (n = 3). (B) Induction of OPN in HeLa and Huh-7 cells during Hyp/RO. Cell extracts prepared from cells exposed to Hyp/RO as in A were analyzed with Western blotting using the indicated antibodies. (C) Sustained level of pAKT in HeLa and Huh-7 cells during Hyp/RO. Cells were left untreated or exposed to Hyp (12 h)/RO (18 h) and Western blot analysis was performed. (D) Expression of OPN DM in HeLa cells increases pAKT level and inhibits caspase-9 activation. HeLa cells were transfected with pcDNA3, OPN WT, or OPN DM expression vector for 24 h and then exposed to Hyp (12 h)/RO (18 h). Cell extracts were analyzed for Western blotting using the indicated antibodies.