(A) Percentage of initial body weight of DT-treated, AgrpDTR/+ mice after subcutaneous implantation of mini-osmotic pumps (mp) loaded with either bretazenil (n = 12) or vehicle (n = 10). DT was injected intramuscularly (im) twice as indicated by arrows. Minipumps were removed on Day 11 after drug solution was depleted.
(B) Liquid diet intake by the mice described in A.
(C) Licking activity of the mice described in A was plotted as average number of licks in 2-h bins. *, p < 0.01, ANOVA.
Error bars represent the standard error of mean (SEM).