Figure 5. Inhibition of GABAA receptors in the PBN or disruption of the neural inputs from AgRP neurons to the PBN produces anorexia.
(A) Percentage of initial body weight of C57BL/6 wild-type mice in which bicuculline (100, 300, and 1000 ng/day) was chronically infused to the PBN area through bilateral cannulae for 4 days and then discontinued.
(B) Intake of liquid diet by the mice described in A. *, p < 0.01, ANOVA between the groups of 100 ng/day and 1000 ng/day. Error bars represent the SEM. N = 5 – 6 mice per group.
(C) Percentage of initial body weight of AgrpDTR/+ and wild-type mice that received a single injection of DT into the PBN area (bilateral, 4 ng per side).
(D) Intake of liquid diet by the mice described in C.