Fig. 3.

Effects of MD on hPXR-, hCAR3-, and hCAR-mediated CYP2B6 reporter gene activation. HepG2 cells were transfected with hPXR (A), hCAR3 (B), or hCAR (C) expression vectors in the presence of CYP2B6-2.2 kb reporter construct. Transfected cells were then treated with vehicle or MD (10, 25, or 50 μM) for 24 h. RIF (10 μM) and CITCO (1 μM) were used as positive controls for hPXR and hCAR, respectively. Luciferase activities were determined and expressed relative to vehicle control. Data represent the mean ± S.D. (n = 3). ***, p ≤ 0.001; **, p ≤ 0.01.