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. 2009 Jun 11;37(9):1887–1894. doi: 10.1124/dmd.109.027854

Fig. 5.

Fig. 5.

Methadone translocates Ad/EYFP-hCAR in human primary hepatocytes. Human hepatocytes (HL8 and HL9) were infected with Ad/EYFP-hCAR as described under Materials and Methods and treated with vehicle, PB (1 mM), or MD (50 μM). After 24 h of treatment, hepatocytes were subjected to confocal microscopy. A, representative Ad/EYFP-hCAR localizations from vehicle control, PB- (1 mM), and MD- (50 μM) treated hepatocytes. B, for each treatment, more than 60 hCAR-expressing cells were counted and classified based on cytosolic, nuclear, or mixed (cytosolic + nuclear) hCAR localizations.