Methadone translocates Ad/EYFP-hCAR in human primary hepatocytes. Human
hepatocytes (HL8 and HL9) were infected with Ad/EYFP-hCAR as described under
Materials and Methods and treated with vehicle, PB (1 mM), or MD (50
μM). After 24 h of treatment, hepatocytes were subjected to confocal
microscopy. A, representative Ad/EYFP-hCAR localizations from vehicle control,
PB- (1 mM), and MD- (50 μM) treated hepatocytes. B, for each treatment,
more than 60 hCAR-expressing cells were counted and classified based on
cytosolic, nuclear, or mixed (cytosolic + nuclear) hCAR localizations.