TABLE 1.
Primer and probe sequences for real-time PCR assays
| Gene | Sequence | Reference |
|---|---|---|
| CYP2B6 | Faucette et al., 2007 | |
| Forward primer | 5-AAGCGGATTTGTCTTGGTGAA-3 | |
| Reverse primer | 5-TGGAGGATGGTGGTGAAGAAG-3 | |
| Probe | 6-FAM-CATCGCCCGTGCGGAATTGTTC-TAMRA | |
| CYP3A4 | Faucette et al., 2007 | |
| Forward primer | 5-TCAGCCTGGTGCTCCTCTATCTAT-3 | |
| Reverse primer | 5-AAGCCCTTATGGTAGGACAAAATATTT-3 | |
| Probe | 6-FAM-TCCAGGGCCCACACCTCTGCCT-TAMRA | |
| UGT1A1 | Smith et al., 2005 | |
| Forward primer | 5-GGCCCATCATGCCCAATAT-3 | |
| Reverse primer | 5-TTCAAATTCCTGGGATAGTGGATT-3 | |
| Probe | 6-FAM-TTTTTGTTGGTGGAATCAACTGCCTTCAC-TAMRA | |
| MDR1 | Maglich et al., 2002 | |
| Forward primer | 5-GTCCCAGGAGCCCATCCT-3 | |
| Reverse primer | 5-CCCGGCTGTTGTCTCCAT-3 | |
| Probe | 6-FAM-TGACTGCAGCATTGCTGAGAACATTGC-TAMRA |