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. 2009 Jul 10;136(15):2545–2555. doi: 10.1242/dev.031781

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Fig. 2. — Otx2 is required for maintenance of the VM Nkx6-1⁺ progenitor domain. (A-X) Representative coronal midbrain sections of E12.5 WT embryos (A,E,I,M,Q,U), En1^+/Cre; Otx2^flox/flox (B,F,J,N,R,V), Nkx2-2^-/- (C,G,K,O,S,W) and En1^+/Cre; Otx2^flox/flox; Nkx2-2^-/- (D,H,L,P,T,X) mutant embryos. (B,D) Loss of ventral Nkx6-1 expression and of Pou4f1⁺ RN neurons in En1^+/Cre; Otx2^flox/flox mice is not rescued in En1^+/Cre; Otx2^flox/flox; Nkx2-2^-/- triple mutants. (F,H) There is a slight reduction of Isl1⁺ OM neurons in both Otx2 mutants. (J,L,N,P) Th/Pitx3⁺ mDA neurons are lost in En1^+/Cre; Otx2^flox/flox but rescued in En1^+/Cre; Otx2^flox/flox; Nkx2-2^-/- mutants. (C,G,K,O) Nkx6-1, Pou4f1, Isl1, Th and Pitx3 expression is unaffected in Nkx2-2^-/- mice. (Q-X) There is a strong reduction of the ventral Nkx6-2⁺ domain (arrows in R,T) but unaltered Dbx1 expression in Otx2 mutants.