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. 2009 Jun 10;136(14):2335–2344. doi: 10.1242/dev.031609

Fig. 1.

Fig. 1.

Expression of Sns and Kirre in embryonic GCNs. (A) Schematic, modified with permission from Hartenstein (Hartenstein, 1993) to indicate the position of GCNs (yellow) at embryonic stage 16. (B-L) GCNs were identified by expression of the sns-GCN-lacZ transgene. (B) Ventral view, stage 16. (C-F) Confocal single section of GCNs at stage 13. Sns (red) and Kirre (red) are present at cell-cell contacts (arrows). Arrow in B indicates GCNs marked by β-galactosidase. (G-L) Ventral views, stage 16. Projections of confocal sections (∼20 μM) (G,J) and single sections (H,I,K,L) reveal Sns (H,I) and Kirre (K,L) on the cell surface (arrowheads), cell-cell contacts (arrows) and in intracellular and cell surface puncta (asterisks). Note the uneven pattern of Sns on the cell surface in (I). (M-T) FACS-isolated nGFP-positive cells from sns-GCN-nGFP transgenic embryos. Single confocal sections show partial co-localization of Sns and Kirre on the surface of mononucleate (9-12 hours AEL) (M-P) and binucleate (12-18 hours AEL) (Q-S) GCNs. Scale bars: 10 μm in C-L; 5 μm in M-T. ES, esophagus; PV, proventriculus.