Table 1.
This table summarizes the exposure parameters investigated in this study along with the CNR and number of insonifications evaluated for each set of parameters. The number of animals column gives the number of locations (one per sequence on a given animal) evaluated for CNR followed by the number of those animals used in histology in parentheses. Each location was insonified for 30 seconds with a PRF of 10 Hz and an unapodized, F/1.5 configuration except the PW Doppler sequence (*) which used an 100 Hz PRF and an apodized, F/4 configuration. The first 8 sequences are presented in the plots herein, while the others serve as discussion points. The mice in row † were insonified with the most aggressive sequence in an additional location, independent of those used for CNR evaluation, for histology purposes only
| # of Animals | Definity Dosage (μL) | Delay after Definity injection (s) | Frequency (MHz) | Pressure (MPapp) | Pulse Duration (ms) | CNR (mean ± SD) |
|---|---|---|---|---|---|---|
| 4(0) | 30 | 0 | 5.7 | 1.1 | 20 | 0 ± 0 |
| 4(2) | 30 | 0 | 5.7 | 6.2 | 20 | 39 ± 8 |
| 4(1) | 30 | 0 | 5.0 | 2.3 | 20 | 22 ± 12 |
| 4(2) | 30 | 0 | 5.7 | 2.7 | 20 | 36 ± 3 |
| 4(2) | 30 | 0 | 6.7 | 3.8 | 20 | 25 ± 8 |
| 4(1) | 30 | 0 | 8.0 | 5.2 | 20 | 42 ± 13 |
| 4(3) | 30 | 0 | 5.7 | 2.7 | 0.002 | 9 ± 4 |
| 4(1) | 30 | 0 | 5.7 | 2.7 | 0.07 | 24 ± 8 |
| 2(0) | 10 | 0 | 5.7 | 6.2 | 20 | 36 ± 2 |
| 2(0) | 60 | 0 | 5.7 | 6.2 | 20 | 41 ± 11 |
| 2(0) | 30 | 7 | 5.7 | 6.2 | 20 | 24 ± 7 |
| 2(0) | 30 | 60 | 5.7 | 6.2 | 20 | 22 ± 1 |
| 1(0) | 30 | 120 | 5.7 | 6.2 | 20 | 29 |
| 2(0) | 30 | 0 | 6.7 | 2.8 | 20 | 23 ± 1 |
| 2(1) | 30 | 0 | 8 | 2.3 | 20 | 0 ± 0 |
| 1(0) | 30 | 0 | 5.7 | 1.6 | 20 | 24 |
| 1(0) | 30 | 0 | 5.7 | 3.8 | 20 | 39 |
| 1(0) | 30 | 0 | 5.7 | 2.7 | 0.00035 | 0 |
| *5(5) | 30 | 0 | 5.7 | 2.7 | 0.007 | 21 ± 9 |
| †0(7) | 30 | 0 | 5.7 | 6.2 | 20 | - |