Figure 8. Morphology of embryonic Purkinje cells in the co-cultures.

(A–D) Elongating and arborizing phenotypes of calbindin-labeled (CaBP, red staining) embryonic Purkinje cells at 7 days in vitro in the postnatal environment in toto (A, B, E17+P0) or co-cultivated with granule cell monolayers (C, D, E17+GR). Embryonic cells were distinguished by postnatal cells on the basis of GFP expression (green staining). Co-expression of calbindin and GFP yielded orange staining. At difference with the E17+P0 condition, in E17+GC co-cultures PCs were growing in contact with DAPI-positive cellular elements (blue) (compare A, B vs C, D). Scale bars, 25 µm.