Table 1. Cellular composition of E17 and P0 cerebellar cultures.
culture type | time point | all cells | Purkinje cells | other neurons | non-neuronal cells |
E17 | |||||
1 DIV | 625.00±90.75 | 123.98±9.95 | 429.25±66.77 | 118.75±26.49 | |
7 DIV | 1143.75±110.10 | 103.42±23.12 | 250.00±31.38 | 783.33±92.08 | |
P0 | |||||
1 DIV | 1027.00±200.50 | 37.50±7.19 | 439.50±60.65 | 539.50±83.79 | |
7 DIV | 1406.67±110.10 | 11.83±0.83 | 435.00±49.90 | 970.00±84.45 |
The table reports the total number of cells/mm2, visualized by DAPI counterstaining, the number of PCs, estimated by counting CaBP-positive cells, of other neurons (CaBP-negative, beta-tubulin-positive cells) and of other non-neuronal DAPI-labeled elements, negative for the tested markers.