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Mismatch of the Ecu534 VP7 sequence with previously published primers. The top sequence is the primer and the bottom sequence is the intended primer binding site in the Ecu534 VP4 sequence. The vertical lines show matches, the asterisks show mismatches, and the colons show partial matches of degenerate bases. The 5’ most base position is shown for the Ecu534 template.
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Santos et al. suggested using primers sBeg9 (Gouvea et al., 1994) and RVG9 (Gouvea et al., 1990) together.
