Figure 1. Plaque formation by HVP2 subtypes in BALB/c, 129 and IFNAR−/− PMDF cultures.
Confluent PMDF monolayers were inoculated at an MOI of 0.25 PFU/cell and photographed at 48 hrs PI. HVP2nv isolates produced complete CPE within 48 hrs in all three cell types. HVP2ap isolates produced discrete plaques by 24 hrs in Balb/c and 129 cell cultures, but these failed to enlarge with time. In contrast, HVP2ap isolates completely destroyed IFNAR−/− cultures by 48 hr PI.