Figure 4.
Bode plots for ionic conductance (at 10 µM of KCl) through the membranes before (black) and after (red) immobilization of ss-DNA (16-mer). Different relative concentrations of the amine and ester (A – G) result in different surface densities of DNA. The effect of DNA binding, shown in H, as the ratio (red squares) of the impedance at 3 kHz before (black triangles) and after DNA binding (blue circles), has the optimal amine/ester ratio for maximal effect near amine:ester = 1:7, as in D. Note that for the surface with maximum amount of amines, the impedance increases after DNA binding.