Table 1.

Primers for Flag-tagged or wide-type PTH1R

Name	Forward or Reverse	Sequence
F1F	Forward	5′-gcg gcg aag ctt gcg atg ggg acc gcc cgg atc-3′
F1R	Reverse	5′-gcg ccc tcg gta cct ctt gtc gtc gtc gtc ctt-3′
F2F	Forward	5′-gac aag agg tac cga ggg cgc ccc tgt ctg ccg-3′
F2R	Reverse	5′-gcg gcg gaa ttc tca cat gac tgt ctc cca ctc-3′

The oligonucleotide primers containing HindIII, KpnI, and EcoRI restriction enzyme sites are underlined. The nucleotide sequence encoding the Flag epitope is shown in bold.