TABLE 2.
R.Y. Results from Each Participating Laboratory for the Polyhistidine- and Polyalanine-Tagged Proteins
| Laboratory | Alanine-tagged R.Y. (%) | Histidine-tagged R.Y. (%) | R.Y. Ratio (His/Ala) |
| 10 | 92.6 | 91.9 | 0.99 |
| 20 | 91.3 | 91.5 | 1.00 |
| 30 | 93.9 | 92.7 | 0.99 |
| 40 | 95.8 | 97.0 | 1.01 |
| 50 | 92.9 | 93.3 | 1.00 |
| 60 | 98.3 | 96.8 | 0.98 |
| 70 | 92.3 | 94.8 | 1.03 |
| 80 | 91.8 | 90.3 | 0.98 |
| 90 | 92.9 | 89.4 | 0.96 |
| 100 | 93.3 | 93.2 | 1.00 |
| 200 | 94.2 | 94.0 | 1.00 |
| 300 | 92.5 | 93.8 | 1.01 |
| 400 | 93.5 | 89.9 | 0.96 |
| 500 | 90.8 | 89.0 | 0.98 |
| 600 | 93.7 | Not reported | – |
| 700 | 100.7 | 84.8 | 0.84 |
| ESRG1 | 95.0 | 91.7 | 0.97 |
| ESRG2 | 90.5 | 92.6 | 1.02 |
| ESRG3 | 94.5 | 97.6 | 1.03 |
| ESRG4 | 93.6 | 94.5 | 1.01 |
| ESRG5 | 91.3 | 105.9 | 1.16 |
| ESRG6 | 90.9 | 95.8 | 1.05 |
| ESRG7 | 94.1 | 91.8 | 0.98 |
| Mean | 93.5 | 93.3 | 1.00 |
| sd | 2.4 | 4.1 | 0.05 |
Raw picomole yield from cycles 1 and 9–17 from the polyhistidine-tagged protein R.Y. calculation. Raw picomole yield from cycles 1–3 and 12–17 were used for the polyalanine-tagged protein R.Y. calculation. R.Y. ratio is the R.Y. of the polyhistidine-tagged protein over the polyalanine-tagged protein. A value of 1.0 means R.Y. values for the two proteins were equal.