Plasmid Vectors and Clones Used in the Study
| DNA construct | Oligonucleotide primer sequence |
|---|---|
| <hGH.cHis.ClaI> | CCATCGATTCCACCATGGCTACAGGCTCCCG |
| <hGH.cHis.AscI> | GGCGCGCCAGAAGCCACAGCTGCCCTC |
| <hGH.nHis.AscI> | GGCGCGCCCTAGAAGCCACAGCTGCCC |
| <hGH.nHis.XhoI> | CTCGAGTTCCCAACCATTCCCTTATCC |
| <HGHpolyA.ClaI.F> | CCATCGATGCTGCAGCTGCAGCTGCAGCTGCATTCCCAACCATTCCCTTATCC |
| <HGHpolyK.ClaI.F> | CCATCGATAAGAAGAAGAAGAAGAAGAAGAAGTTCCCAACCATTCCCTTATCC |
| <HGHpolyY.ClaI.F> | CCATCGATTACTACTACTACTACTACTACTACTTCCCAACCATTCCCTTATCC |
| Cycle # | |||||||||||||||||
| Sample | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| C | F | P | T | I | P | L | S | R | L | F | D | N | A | M | L | R | A |
| H | K | H | H | H | H | H | H | H | H | L | E | F | P | T | I | P | L |
| A | K | I | D | A | A | A | A | A | A | A | A | F | P | T | I | P | L |