FIGURE 3.

A comparison of aphid protein extracted by the TCA-acetone, phenol, and multi-detergent techniques using 2-DE. The 24-cm, 12% SDS-PAGE gels (Jule, Inc.) were stained with Colloidal blue and visualized using the Typhoon Variable Mode Imager (GE Healthcare). (a) TCA-acetone extraction, (b) phenol extraction, (c) technical replicate 1 of the multi-detergent extraction, (d) technical replicate 2 of the multi-detergent extraction. The TCA-acetone extraction seemed to perform better with acidic proteins, and the phenol extraction method seemed to perform better with proteins from a neutral to basic pI. As the 1-D gel suggested, multi-detergent technical replicates had distinct 2-D gel patterns.