TABLE 2.
Identity of select, highly abundant proteins, unique or shared in the three-protein extraction techniques
| Protein | Genbank accession of top BLAST hit | MASCOT score (MASCOT E value) |
MW Observed | pI Observed | ||
|---|---|---|---|---|---|---|
| TCA-acetone | Phenol | MD | ||||
| Calreticulin | gi:66545506 | 353(3.3e-30) | Not found | 406(1.6e-35) | 55.6 KD | 4.25 |
| Bicaudal | gi:187121188 | Not found | 466(1.6e-41) | Not found | 25 KD | 5.55 |
| Bicaudal | gi:187121188 | Not found | Not found | 478(1e-42) | 25 KD | 4.0 |
| Cyclophilin | gi:156536889 | Not found | 214(2.6e-16) | Not found | 18 KD | 9.7 |
| HSP60 | gi:6066606 | 939(8.2e-89) | Not found | Not found | 66.2 KD | 5.15 |
| p0 ribosomal protein | gi:187117172 | 474(2.6e-42) | Not found | Not found | 39 KD | 5.65 |
| Laminin B2 | gi:17737557 | 97(1.4e-4) | Not found | 112(4.1e-06) | 180 KD | 4.8 |
| Mitochondrial ATP synthase α subunit precursor | gi:52630965 | Not found | 1100(6.5e-105) | Not found | 60 KD | 8.6 |
| Enolase | gi:5839036 | 744(2.6e-69) | 531(5.2e-48) | 545(2.1e-49 | 55 KD | 5.7 |
| Enolase | gi:5839036 | Not found | 747(1.3e-69) | Not found | 55 KD | 5.6 |
| Enolase | gi:5839036 | Not found | 535(2.1e-48) | Not found | 55 KD | 5.5 |
MD=Multi-detergent; HSP60=heat shock protein 60.