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. Author manuscript; available in PMC: 2009 Aug 20.
Published in final edited form as: Inflamm Bowel Dis. 2008 Jul;14(7):898–907. doi: 10.1002/ibd.20414

FIGURE 8.

FIGURE 8

Resistance to campto-thecin-induced caspase-3 activation and apoptosis in cells expressing dominant-negative Gi2-alpha. T84 (A) and RKO (B) cells expressing control vector alone (Neo) or G203T- Gi2-alpha were treated with 5 µM camptothecin for 6 hours alone, or following overnight pretreatment with 20 µM ar-achidonicacid. The data shown are the mean ± SD of triplicate caspase-3 activity measurements and are representative of 3 separate experiments. C: 100 µg of the cell lysates from control and CPT-treated cells at 0,3,6, and 24 hours were separated and blotted with anti-activated caspase-3 Ab (recognizes 19 and 17 kDa fragments). D: CPT-treated Neo and Gi2-alpha-expressing cells were evaluated for apoptosis by TUNEL staining; the data represent the mean ± SD percentage of TUNEL+ cells in 10 medium-powerfields.**P < 0.001.